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Description
Cell pellets were resuspended in 500 l of IP buffer 1 [50 mM HEPES pH 7.6, 125 mM KCl, 0.1% NP-40, 20% glycerol, 1 mM EDTA, 1 mM PMSF, and Complete protease inhibitor cocktail (Sigma)] and disrupted using Mini-Beadbeater-16 (BioSpec Products)

Highly sensitive and practical fluorescent sandwich ELISA for ciguatoxins

doi: 10.1097/TP.0000000000002049 29 BronkhorstAJUngererVHoldenriederS

Additionally, the encapsulation of hydrophobic RES within hydrophilic DS-CS nanoparticles enhances cellular absorption and bioavailability [98]
