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Description
Strains of BL21 and its derivatives, or another protease deficient strain, are recommended for maximal protein expression
Quantitative PCR was performed via the use of primers for the human target genes involved in iron homeostasis, ferroptosis, and inflammation processes (Supplementary Tables 1 and 2) with GoTaq qPCR Master Mix (Promega) via a Quant Studio 5 PCR System (Thermo Fisher Scientific)
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2 , e142e152 (2020)
