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Using a human-relevant mouse model of metastasising breast cancer, in which experimental study, breast carcinoma cells are injected into pre-cleared mammary fat pads (orthotropic transplantation), activation of GRs at distant metastatic sites was found to be required for colonization and heterogeneity of metastases, as well for reduced survival of transplanted mice [22]

[DOI] [PMC free article] [PubMed] [Google Scholar] 99.Osawa F., Fujii T., Nishida T., Tada N., Ohnishi T., Kobayashi O., Komeda T., Yoshida S

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Images were captured under an inverted epifluorescence microscope (Zeiss Axio Observer Z1, Oberkochen, Germany) and processed in ImageJ software 1.52a (NIH)