glutathione detection review Glutathione-mediated nanomedicines for cancer diagnosis and therapy Recent progress in electrochemical detection

The scaling problem: Translating 5 mg/kg from mice to humans is complex

Final Thoughts Your TRT dosage guidelines work only when you commit to three essentials: starting conservatively, maintaining consistent administration, and following through with regular blood work

Higbie V, LaPelusa MB, Shah P, Childress M, Gasco A, An A, Bent AH, Kee BK, Kopetz S , Le P, Morris VK, Pant S, Parseghian C, Shen JP, Wolff RA, Overman MJ

Case Studies Case 1: TEV Won't Cut Protein: 45 kDa enzyme Construct: His6-TEV site-Target Problem: No cleavage after 24 hours with excess TEV Diagnosis: Crystal structure of homolog showed N-terminus buried in a groove TEV site was inaccessible to protease Solution: Redesigned: His6-(GGGGS)-TEV site-(GGGGS)-Target Cleavage now complete in 4 hours Case 2: Thrombin Destroys the Protein Protein: 30 kDa signaling domain Construct: His6-Thrombin site-Target Problem: Multiple bands after cleavage