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10.1007/s11302-014-9428-1 4 AlexanderJ.ChangG
In both lower and higher eukaryotes, the initiation of autophagy requires the assembly of specific protein complexes: (1) the Atg1-Atg13-Atg17 (ULK1-Atg13-FIP200 in mammals) kinase complex, which initiates phagosome formation at the phagophore assembly site (PAS, an Atg8-rich cytoplasmatic site) (Kabeya et al., 2005
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For adipogenic differentiation, Oil Red O staining demonstrated the presence of lipid vacuoles within the cytoplasm of hGMSCs, indicative of adipocyte formation
