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The A53T mutation in the -synuclein gene ( SNCA A53T , or A53T) is one of the key missense gene mutations linked to early onset familial PD, as the mutated -synuclein variant is prone to self-aggregation (12)

Bacteriostatic water itself is the standard diluent used in peptide reconstitution because its 0.9% benzyl alcohol content inhibits bacterial growth across multiple vial entries, which is why its specified over plain sterile water in nearly all peptide handling literature a distinction covered in depth in our guide on bacteriostatic water vs sterile water for peptides

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We further defined the statistics both in the materials & methods part as well as in the figure legends