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Description
Briefly, cells were lysed in the lysis buffer described above, and loaded onto a stepwise sucrose gradient composed of 700-l layers of sucrose of increasing percentage (top-to-bottom: 5%, 10%, 15%, 20%, 25%, and 30%)
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Metabolic signaling in T cells
Zhang Y, Murugesan P, Huang K, Cai H (2020) NADPH oxidases and oxidase crosstalk in cardiovascular diseases: novel therapeutic targets
