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Its mechanism of action involves the bidirectional regulation of bone tissue remodeling: promoting bone formation-related signaling pathways on one hand, and inhibiting osteoclast-mediated bone resorption on the other

By doing so adverse effects can be measured

After incubation at 37 C with shaking (500 rpm) for 1 h., the cells were pelleted at 5,000 x g for 10 min at 4 C, washed once with ice-cold phosphate-buffered saline (PBS, pH 7.4), resuspended in 200 L of cold TE lysis buffer (30 mM TrisHCl, 1 mM EDTA, pH 8.0), and transferred to Lysing matrix B tubes (MP Biomedical)

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