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210,217 The dephosphorylation of occludin might partly occur as a result of protein phosphatase (PP)2A activity
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The following day, cells were washed three times with DPBS and incubated with the corresponding secondary antibodies conjugated to Alexa Fluor 488 or Alexa Fluor 555 fluorophores (Thermo Fisher Scientific, Inc.) for 2 h at room temperature
conjugation with amino acids and acetylation reaction
