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Description

Recombinant lentiviruses were produced by transfecting HEK293T cells with pCMV-VSV-G (VSV-G protein), pCMV-dR8.2 (lentivirus packaging vector) and shRNA-expressing constructs using Lipofectamine 2000 transfection reagent as previously described

This is because E2 production by the follicles in the ovaries is inhibited due to the inhibitory effect of the COC on follicle stimulating hormone (FSH) production, which normally results in follicular maturation