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During gavage mice were under light anesthesia to limit discomfort (3% isoflurane at a 1.5 L/min O 2 flow rate for ~1 min)
Freezing can cause the peptide molecules to aggregate irreversibly, meaning they clump together and cannot be separated again
36 The mTOR system contains rapamycin-sensitive complex 1 (mTORC1) and rapamycin-insensitive complex 2 (mTORC2)
