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Description
This colorimetric assay determines relative protein concentration by comparison to a bovine serum albumin (BSA) protein standard, from which a calibration curve was generated

& Mieyal, J

Depending on the redox state of the free cysteine residue (Cys34), HSA exists in both reduced and oxidized forms 14,19

They compared the effects of cysteine proteolytic enzyme inhibitors, necrosis inhibitors, and ferroptosis inhibitors on the activity of human retinal pigment epithelial cell line (ARPE-19) after exposure to 500 mol/L tert-butyl hydroperoxide (TBH) in vitro
