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MTX inhibits key enzymes of folate metabolism, including DHFR, thymidylate synthase, MTHFR and SHMT (57,58)

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doi: 10.1152/ajpgi.1995.269.4.G467

Comparison between groups was made using one-way analysis of variance (ANOVA) followed by post hoc Tukey HSD test or non-parametric Kruskal-Wallis test for multiple independent samples with post hoc test by the methods of Conover
