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Methods Cell culture and growth conditions All cell lines were cultured in either RPMI (Gibco, 11875119) or DMEM media (Gibco, 12430063) supplemented with 10% fetal bovine serum (Gibco, 12483020) and 1% penicillin-streptomycin (Gibco, 15140-122) and passaged by washing with Dulbeccos phosphate buffered saline (PBS) (Gibco, 14190250), and incubating with trypsin-EDTA (0.25%) (Gibco, 25200114)

At the outer membrane/cytosol interface, H 2 O 2 traverses peroxiporins (e.g., AQP8/3), enabling communication with cytosolic kinases and phosphatases without causing bulk oxidative stress (da Silva et al

Also, it is a key enzyme involved in one of the central pathways of one-carbon metabolism and is essential for various cellular functions, including DNA synthesis, methylation reactions, and the production of important molecules such as SAM

Farhat Z, Browne RW, Bonner MR, Tian L, Deng F, Swanson M, Mu L (2018) How do glutathione antioxidant enzymes and total antioxidant status respond to air pollution exposure