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Description
After washing the membrane with TBST with residual secondary antibody (Diluted at 1:2000), protein bands were performed by Bio-Rad ChemiDoc XRS+
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Plasma TMAO concentrations were analyzed in-house, while complete blood count, blood urea, creatinine, and estimated glomerular filtration rate (eGFR) were analyzed by Norfolk and Norwich University Hospital Pathology Department (Norwich, UK)
