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Description
DNA sequences encoding VH and VL domains are amplified from animal cDNA libraries (e.g., human or murine cDNA libraries of lymphoid tissues)

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Specifically, factoring in chamber temperature and humidity, the cycle analyzers filtered the acquired signals, and BUXCO algorithms (Fine Pointe) generated an array of box flow data that identified a waveform segment as an acceptable breath

The fractions containing the recombinant proteins were pooled and dialyzed by ultrafiltration to remove salt and loaded onto a DEAE-cellulose column (Sigma Aldrich) equilibrated with a 30 mM Tris-HCl pH 8.0 and 1 mM EDTA buffer
