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After conversion, a 5 5 median convolution filter was applied to sharpen the border of the converted phasor plot, decreasing the variance of phasor distribution (Figure 2A), as described in reference ( FIGURE 2 As a reference point, we imaged transgenic worms that express DHS-3::GFP and VIT-2::GFP (Figures 2BD) with high-concentration (5 M) Nile Red vital staining, the worm with low-concentration (50 nM) Nile Red vital staining (Supplementary Figure S3 left), as well as Nile Red in water and lipid solution as an in vitro reference (Supplementary Figure S4A)

P.RelanoP.et al (2004)

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Jagust WJ