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DOI: 10.1074/jbc.M112.405738 45
For library preparation and sequencing, library construction was performed using a SENSE 3 mRNA-Seq Library Prep Kit (Lexogen, Inc., Austria) according to the manufacturers instructions
Abstract This chapter describes the use of glutathione S-transferase (GST) gene fusion proteins as a method for inducible, high-level protein expression and purification from bacterial cell lysates
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