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165,166 Based on the structure and the enzymatic activity of their periplasmic domains, HMW-PBPs are further assigned to two classes: class A PBPs (aPBPs), which carry both glycosyltransferase and transpeptidase activities, and class B PBPs (bPBPs), which only have transpeptidase activity

One difficulty in understanding the pharmacology of bivalent and multivalent ligands is discerning whether the effects are from a cooperative synergistic binding mode or due to the effect of increasing the pharmacophore concentration without synergy effects