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Briefly, HEK293T packaging cells were transfected with control (firefly luciferase, GFP or shGFP) or target gene-expressing pLEX and pLenti constructs or pLKO shRNA constructs, together with pCMV-VSVG and pCMV-8.2 lentiviral packaging plasmids

Pituitary (2002) 5:2436

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Additionally, patients who could become pregnant must have monthly pregnancy tests and agree to abstain or use two methods of birth control, as taking Accutane during pregnancy can cause severe birth defects