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Crystallisation of CsiD-ligand complexes Purified CsiD protein was concentrated to 13 mg/mL and its crystallization in liganded form pursued by co-crystallization, crystal soaking or a combination of both approaches, as follows: Apo CsiD: crystals grew from reservoir solutions containing 80 mM sodium chloride, 12 mM potassium chloride, 20 mM magnesium chloride hexahydrate, 40 mM sodium cacodylate trihydrate pH 6.0, 30% [v/v] 2-methyl-2,4-pentanediol, 12 mM spermine tetrahydrochloride

J Electroanal Chem