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Further pharmacological evaluation of a novel synthetic peptide bradykinin B2 receptor agonist

doi:10.1002/jcp.1041330217 27

Combination with immunotherapy (e.g., anti-PD-1) promotes immunogenic ferroptosis by increasing the infiltration of CD8 + T-cells, while combination with chemotherapy (e.g., 5-fluorouracil) increases chemosensitization in CRC cells (128)

To construct the sorting vector for gene overexpression, we amplified the whole expression cassette of N-signal-GST-EGFP-GPI DAF and N-signal-GST-EGFP-GPI CEAM7 from pEGFP-GST-GPI DAF and pEGFP-GST-GPI CEAM7 vectors and joined them with PCR linearized pcDNA3.1 vector using ClonExpress II One Step Cloning Kit