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Description
J Biol Chem 286(10):80758084

In Gclm KO mice, we have identified a vicious feed-forward process between OxS and neuro-inflammation occurring early during brain development, which underlies the long-lasting effect on PV neuron/PNN integrity [90]

Three critical strategies considered are: (1) carbon sources in medium to enhance the nitrogenase activity of NG13, (2) genetic modifications to NG13 to increase the carbon flux to 2-OG, a direct precursor of glutamate, and (3) culture volume and vessel optimization to increase aerial nitrogen supply to cells

Add one at a time to identify any individual responses or issues
