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doi: 10.1371/journal.pone.0045499 20 KuoCYJMurphyRRupenthalIDMugishoOO

Beijnen JH, Potman RP, van Ooijen RD et al

Its anxiolytic potential operates through a completely different pathway: the reduction of systemic inflammation that drives neuroinflammation and, consequently, anxiety

Transduced cells were selected with 2 g/ml puromycin (Sigma-Aldrich, TR-1003-G).The DNA sequences of these shRNA genes were as follows: sh-NRF2-a forward: 5- CCG GCT TGC ATT AAT TCG GGA TAT ACT CGA GTA TAT CCC GAA TTA ATG CAA GTT TTT G-3 and reverse, 5- AAT TCA AAA ACT TGC ATT AAT TCG GGA TAT ACT CGA GTA TAT CCC GAA TTA ATG CAA G-3, sh-NRF2-b forward: 5-CCG GAG AGC AAG ATT TAG ATC ATT TCT CGA GAA ATG ATC TAA ATC TTG CTC TTT TTT G-3 and reverse, 5- AAT TCA AAA AAG AGC AAG ATT TAG ATC ATT TCT CGA GAA ATG ATC TAA ATC TTG CTC T-3, sh-C forward: 5- CCG GGC AAG CTG ACC CTG AAG TTC ACT CGA GTG AAC TTC AGG GTC AGC TTG CTT TTT G-3 and reverse, 5- AAT TCA AAA AGC AAG CTG ACC CTG AAG TTC ACT CGA GTG AAC TTC AGG GTC AGC TTG C-3