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1B) and an increase in the myotube diameters (Fig

[DOI] [PMC free article] [PubMed] [Google Scholar] 6.Wang D.D., Mao Y.Z., He S.M., Yang Y., Chen X
One milliliter of a PBS-based reaction mixture containing propidium iodide (20 g/mL), RNase (0.2 g/mL), and 0.1% Triton X-100 (Sigma) was added to the cell samples at a density of 1 10 6 cells/mL and incubated in the dark for 40 min before a DNA content analysis was conducted through flow cytometry

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