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Lysates were collected and centrifuged at 17,000 rpm for 5 min at 4 C to pellet cell debris and then added into HEGA buffer for High-Performance Liquid Chromatography (HPLC) analysis

These include the deficiencys severity, underlying medical conditions, and how well the patient responds to treatment

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The association between micronutrient status and sleep quality in patients with depression: a case-control study