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The insoluble portion was subsequently removed by centrifugation and the supernatant was incubated with Ni-NTA resin (GE Healthcare) for 2 h batch binding at 4 C

Typical dosing protocols used in research settings have varied considerably, with reported ranges from 5-10 mg administered subcutaneously for periods of 10-20 days, often followed by several months without treatment

Since he was 40 years old when he flared and his BMI was low, the risk of having elevated VEGF was high [43] possibly making him a poorer candidate for the effects of a high FA dose

The column was washed with 50 mM Tris-Cl, pH 8.0 and 250 mM NaCl