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The onset of AF correlates with the senescence of ECs and fibroblasts ( 3.3 Ageing in vascular smooth muscle cell VSMCs are vital for regulating vascular wall tension and maintaining blood pressure ( Telomere shortening, DNA damage, oxidative stress, and autophagic dysfunction can all cause VSMC senescence ( Interestingly, the replicative senescence of VSMCs mediates their phenotypic transformation through runt-related transcription factor-2 (RUNX-2) and induces age-related medial arterial calcification ( Like other heart cells, VSMC senescence also leads to CVDs, most commonly AS

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Furthermore, under inflammatory conditions, AD-MSCs display more pronounced proliferation enhancement and simultaneous chondrogenic inhibition compared to amniotic fluid-derived MSCs (AMSCs) ( Besides cytokines, matrix metalloproteinases (MMPs) also play a key role in the inflammatory microenvironment