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The antimicrobial activities and the mode of action of the first equine -defensin DEFA1 were investigated

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Blood should be drawn into appropriate tubes, left to coagulate for 30 minutes at room temperature, centrifuged at 2,000 times gravity for 10 minutes, and stored at minus 80 degrees Celsius within one hour of collection

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Neutrophils contain an extensive antimicrobial arsenal that includes a) Pentraxin 3 contained in specific granules and neutrophil extracellular traps, b) elastase (a component of azurophil granules), and c) nicotinamide adenine dinucleotide phosphate hydrogen (NADPH) oxidase

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Western blot Cells were harvested and proteins were extracted using cell lysis buffer supplemented with 0.3% phenylmethylsulfonyl fluoride and proteinase and phosphatase inhibitors

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