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Additionally, another mTOR-KI, AZD8055, potently induces autophagy and inhibits proliferation in a broad range of cancer cell lines (108)
Oxidative stress reduces glycolytic flux by oxidation of the active site cysteine of the glycolytic enzyme glyceraldehyde-3-phosphate dehydrogenase, which inactivates the enzyme within seconds of oxidative stress, or in response to endogeneous increases in oxidant levels, for instance, upon anchorage-independent growth of cancer cells 23,3437
Age also plays a role in research design
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