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Membranes were then incubated with one of the following primary antibodies overnight at 4 C: AQP3 (1:1000, Santa Cruz Biotechnology, TX, USA), AQP4 (1:1000, Abcam Biochemicals, Grand Island, NY, USA), claudin-1 (1:1000, Invitrogen Technology, Carlsbad, CA, USA), occludin (1:1000, Invitrogen Technology, Carlsbad, CA, USA), Beclin-1 (1:1000, Cell Signaling Technology, Danvers, MA, USA), Atg5 (1:5000, Abcam Biochemicals, Grand Island, NY, USA), iFABP (1:1000, Santa Cruz Biotechnology, TX, USA), HSP70 (1:1000, Enzo Life Sciences, Inc., NY, USA), LC3 (1:1000, Sigma Aldrich) or -actin (1:5000, Sigma Aldrich)

Front Public Health 11 , 1107692 (2023)

Blood tests help confirm deficiency, monitor the effectiveness of injections, and prevent unnecessary overuse