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Furthermore, delphinidin restores the expression of downregulated MicroRNA-204-3p, which inhibits the V3-integrin/FAK signaling pathway to further suppress EMT and metastasis (Huang et al., 2019)

Briefly, BAT was homogenized in 10% w/v of ice-cold STE buffer (0.25 M sucrose, 1 mM EDTA, 10 mM Tris-HCl, pH7.4) by 10 strokes of the loose-fitting pestle of the Dounce homogenizer

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This means that if alcohol has been consumed within 48 hours of surgery, general anaesthetic and other sedatives may not be as effective
