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Why gradual titration matters Semaglutide works through multiple mechanisms
Insomnia was confirmed by monitoring sleep disturbances and alterations in neurotransmitter levels using high-performance liquid chromatography (HPLC)
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Statistical analysis For animal studies, sample sizes ( n = 5 per group, 3 in the tirzepatide-treated group) were determined based on prior in-house experience and previous studies using similar mouse models to ensure adequate power to detect biologically relevant effects
