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57: 19649 Jain S, Hreczuk-Hirst DH, McCormack B, et al

The weight loss itself improves metabolic function, potentially reducing the dose required to maintain results

Excess B vitamins are excreted by the kidneys through the urine, causing bright yellow urine

Typically, cells were seeded in 100 L RPMI phenol red free media per well in duplicates on a black 96-well plate with clear bottom (Thermo Scientific)