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According to the FDA labeling for bulk pharmacy packages, these containers are designed to be punctured only once with a sterile transfer device to distribute the water into multiple single-use vials immediately

Ice crystals that form during freezing physically shear peptide bonds, similar to the damage caused by vigorous shaking

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Step-by-Step Reconstitution Step 1: Clean Everything Protocols describe wiping the tops of both vials (MOTS-C and BAC water) with alcohol swabs, then letting them air dry for 10 seconds