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A 2nd 100 l of 80 % methanol (80 C) was added to the pellet, the sample was vortexed, centrifuged at 13,000 g for 30 min at 4 C, and the 2nd 100 l of supernatant was saved

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Doxycycline induction of the RNAi- gstA2 hairpin in vegetative log phase cells reduced gstA2 transcripts in AX4 cells, and did not significantly affect levels of other gstA transcripts (Fig 7A and 7B), indicating specificity of the targeted construct to suppress the endogenous DdGSTA2 enzyme expression by as much as 80% when compared to uninduced cells (Fig 7C7E)

Gut microbiota functions: metabolism of nutrients and other food components