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doi:10.1371/journal.pone.0029817
Then, the cells were lysed on ice in 800 l of lysis buffer 1, which was composed of 7 M urea (J.T.Baker), 30 mM tris(hydroxymethyl)aminomethane (Sigma-Aldrich), 2 M thiourea (Sigma-Aldrich), 0.1% Triton X-100 (Sigma-Aldrich), protease inhibitors (EDTA free cocktail tablet, Roche Diagnostics, added according to the manufacturers instructions) and phosphatase inhibitors [1 mM sodium orthovanadate (Sigma-Aldrich), 9.5 mM sodium fluoride (J.T.Baker) and 1 M okadaic acid (Sigma-Aldrich)]
10.1053/j.gastro.2012.05.051 89
5 fold higher with EGCG/AA NPs
