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During the demultiplexing step, the removal of adapters (crucial for preparing the reads for alignment to a reference genome, as adapter sequences can interfere with alignment accuracy) and barcodes (to ensure that only the actual RNA sequences of interest are retained for each sample, preventing sample contamination) will be performed with Porechop (v0.2.4 + galaxy1)

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doi: 10.3390/nu10020252

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