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In addition, the supplementation of a mixture of amino acids (arginine, lysine, and histidine) at 0.1, 1, or 10% in the amniotic fluid on e13 increased body weight and the relative weight of the heart, kidney, liver, and small intestine at hatch (193)

Antioxidants Antioxidants such as edaravone, which lowers ROS levels, and ebselen, a glutathione peroxidase mimetic, have shown promising results in studies involving kidney disease models, improving renal function, lowering lipid peroxidation, and increasing endothelial and epithelial cell survival (126)

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After establishing this line, it was grown and expanded further in RPMI 1640 media supplemented with 10% fetal bovine serum