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MerrillJTWallaceDJWaxSKaoAFraserPAChangPet al

The gel was fixed for 30 min in fixation buffer (isopropanol 25%, acetic acid 10%), stained for 2 h with Coomassie 250 (60 mg/L) for 30 min followed by overnight destaining in acetic acid 10%, then the gel was dried

LD, loading dose

Individuals with severe liver disease (such as cirrhosis or hepatic encephalopathy) or severe kidney disease should not take L-Glutamine, as their organs may be unable to effectively clear the resulting ammonia, leading to dangerous neurotoxicity
