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The expression of mRNAs for hepatocyte growth factor/scatter factor, its receptor c-met, and one of its activators tissue-type plasminogen activator show a systematic relationship in the developing and adult cerebral cortex and hippocampus
Immunoblotting The dishes were washed 2 with ice-cold PBS, lysed in 1 Laemli sample buffer, and subjected to standard SDS-PAGE using separate 4%12% Bis-Tris gels, after which the proteins were overnight transferred at 4C to polyvinylidene fluoride membranes
Create a Sterile Field: Clean your work surface thoroughly
