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At a minimum, getting the recommended dietary allowance (RDA) for vitamin C and vitamin D is necessary for the immune system to function properly
Upon antigen encounter, successful activation triggers a metabolic shift: Effector T cells upregulate aerobic glycolysis (the Warburg effect) to rapidly generate biosynthetic precursors (nucleotides, amino acids, lipids) necessary for clonal expansion and cytokine production, while still maintaining OXPHOS capacity
This method can therefore give somewhat lower reduction states than the spectrophotometric assay (eg, Queval et al., 2011)
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