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Li et al demonstrated that vitamin B12 may up-regulated insulin like growth factor-1 (IGF-1) gene expression, and showed a better neuroprotective effect in the presence of good control of hyperglycemia in rats with diabetic peripheral neuropathy 20

After 9 h incubation, the supernatants were collected by centrifugation and the release of ATP was detected by the luminescent ATP detection assay kit as described in the manufactures protocols

mTORC1 and ferroptosis: regulatory mechanisms and therapeutic potential
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