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The most common types of ROS damage in organ preservation stem from 1) the breakdown of purines during hypoxia (e.g., in static cold storage) and/or 2) excessive superoxide production from electron leakage from the electron transport chain during reperfusion (i.e., the re-introduction of blood flow), resulting in excessive hydrogen peroxide production, glutathione (GSH) depletion, and mitochondrial dysfunction

doi: 10.1016/j.cca.2020.05.041

-Lipoic acid reduces infarct size and preserves cardiac function in rat myocardial ischemia/reperfusion injury through activation of PI3K/Akt/Nrf2 pathway

GSH treatment decreased MitoSOX fluorescence and cytoplasmic intracellular calcium levels (Fig