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Consistently, knockdown of Usp2 did not mitigate the alterations in ferroptosis-related markers induced by TFRC OE upon TGF1 (Fig
Quantification of live cells Flow cytometry was performed using the CellEvent Caspase-3/7 Green Flow Cytometry Assay Kit (Thermo Fisher Scientific) and SYTOX blue dead cell stain (Thermo Fisher Scientific) on a FACS Verse system (Becton, Dickinson and Company
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Invest Ophthalmol Vis Sci 59(9):19601960 Kaiser PK, Boyer DS, Campochiaro PA, Heier J, Kornfield J, Kuppermann BD et al (2017) Topline results from prospective, double-masked, placebo controlled phase 2b clinical study evaluating Luminate in patients with diabetic macular edema
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549,550,551,552,553 Single-cell RNA sequencing revealed a significant reduction in astrocytes in AD mice, with ferroptosis activated in astrocytes, and FTH1 in astrocytes was identified as a hub gene associated with ferroptosis