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The medium was replenished with medium containing antibiotic 24 hours post-transfection

ROS Is Involved in Ototoxicity Induced by Aminoglycosides It is generally accepted that ROS is involved in ototoxicity induced by aminoglycosides, and antioxidants can mitigate the effects of ototoxicity (Xie et al., 2011)
This process functions to limit growth and protein synthesis when nutrient availability is compromised