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Compound structure is represented by tethered triangles and a cycle.(BD) Immunoblotting assays showing the BRD4 protein levels in HCC1806 or 22Rv1 cells after incubation with vehicle, BRD4 inhibitor (+)-JQ1 (C), BRD4 PROTAC MZ1 (CE), optimized MZ1 analogs MZ1-Cs-Na (C), MZ1-Cs-NH (D), or MZ1-Cs-NB (E) under indicated conditions.E: Tumor volume after vehicle, MZ1, or MZ1-C14-Na treatment.G: Tumor volume after vehicle, MZ1, or MZ1-C12-NB treatment

It affected vascular senescence and atherosclerosis by activating SIRT1, deacetylating FOXO3a, and modulating PGC1 (Wang J

We can thus confirm that GST-Rec14-His and Rec14-His were not completely cleaved and removed from the GST beads (lane 11)