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Specifically, blood was drawn into a pre-cooled serum test tube wrapped in a cold gel bag, mixed, and allowed to clot in cold at least 60 minutes

Furthermore, membranes were washed with PBS-T and incubated overnight at 4 C with primary antibodies for TH at dilution of 1:1000 (Thermo Fisher Scientific, #OPA1-04050) and the sites of antibodyantigen reaction were visualized with horseradish peroxidase-conjugated secondary antibodies (P448, diluted 1:3000

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Roles for vitamin K beyond coagulation