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In this regard, NAD+ is a substrate for the deacylation reactions catalyzed by sirtuins, particularly sirtuin 1 and 3 (SIRT1 and SIRT3)

Cell Culture Human keratinocytes (HaCaT cells) and mouse-derived melanoma cell lines (B16F10), which were used for in vitro studies, were obtained from ATCC (Manassas, VA, USA)
