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Ivan M, Kondo K, Yang H et al (2001) HIFalpha targeted for VHL-mediated destruction by proline hydroxylation: implications for O2 sensing

To investigate whether the formation of PIF4 nuclear speckles is affected by ambient temperatures, we expressed mGFPmut2-tagged PIF4 in Nicotiana benthamiana leaves through Agro-infiltration and compared nuclear speckle formation under cooler (20 C) and warmer temperatures (27 C)

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